Lensless imaging enables cost-effective phage therapy diagnosis

With the increasing number of drug-resistant bacterial infections worldwide, new interest in phage therapy is increasing. The WHO warns of a “slow tsunami” of antibiotic resistance, which could kill 10 million people annually from antibiotic-resistant infections by 2050.

Based on the use of personalized cocktails composed of highly specific bacterial viruses, phage therapy uses bacteriophage, a type of virus, to treat pathogenic bacterial infections. Following clinical studies of promising phage therapy to treat burn infections, urinary tract infections, and other problems caused by antibiotic-resistant bacteria, scientists say there is a synergistic effect between phage and antibiotics. There is growing consensus among them.

Phage therapy, first performed in 1919, relied on various tests on agar to determine the most active phage for a particular bacterial target or to isolate new lytic phage from environmental samples. To do. However, these culture-based technologies need to be interpreted through direct visual detection of plaque.

In a recent research article, “Phage susceptibility testing and determination of infectious titers by wide-field lensless monitoring of phage plaque growthThe team reported a lensless technique for testing bacterial susceptibility to phages on agar and measuring infectious titers.

In addition to CEA-Leti, the team includes the CEA-Grenoble Interdisciplinary Institute (CEA-Irig), the Center National de la Recherche Scientifique-Laboratoire des Technologies dela Microélectronique (CNRS-LTM), and the Phage Therapy Team at Lausanne University Hospital in Switzerland. Well known in French as CHUV.

The team has a custom-designed wide field of view that allows continuous monitoring with a very large area sensor (8.64 cm2), in addition to investigating computer-assisted methods to facilitate and accelerate phage therapy diagnosis. I studied phage plaques using a lensless imaging device. ).

“We report bacterial susceptibility to anti-Staphylococcus aureus phage in 3 hours and estimate infectious titers in 8 hours and 20 minutes,” the paper explains. “In pre-phage therapy susceptibility testing, macroscopic observation and phage plaque counting are still the most widely used techniques, resulting in much shorter results than previously required 12-24 hours. In addition, continuous monitoring of the sample will allow us to study the growth rate of plaques and gain a deeper understanding of the interaction between phages and bacteria. “

With a lensless demonstrator at a resolution of 4.3 μm, scientists also detected phage-resistant bacterial microcolonies of Klebsiella pneumoniae within the boundaries of the phage plaque.

“This shows that our prototype is also a good device for tracking phage resistance,” said Pierre Marcoux, a scientist at CEA-Leti’s Microtechnology Division for Biology and Health. Stated. “Therefore, lensless imaging is an all-in-one method that can be easily implemented in a cost-effective and compact device at the Phage Laboratory to assist in the diagnosis of phage therapy.”

https://www.electronicsweekly.com/news/business/lensless-imaging-enables-cost-effectivephage-therapy-diagnosis-2021-04/ Lensless imaging enables cost-effective phage therapy diagnosis

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