Examine the small cells that surround the smallest blood vessels in the brain

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One of the laboratories of the Institute of Basic Medicine has equipment equivalent to millions of kroners. Few research groups have the resources to buy an electron microscope when conducting scientific research. Using an electron microscope, Professor Mahmood Reza Amiry-Moghaddam and his colleagues can examine intracellular microstructures.

“We’re talking about nanometers and micrometers here,” explains Amiry-Moghaddam of the Department of Molecular Medicine.

“You can see the small structures of cells and use immunohistochemical methods to detect where membrane proteins are and whether they can move from one domain to another. You can’t go deep into the cell! “

The Amiry-Moghaddam group celebrated yet another contribution to the advancement of scientific knowledge.This time, in an article published in the journal Nature Communications..

University of Oslo at the forefront

The University of Oslo has been assisted by many international research teams for its electron microscopy. However, the expertise of scientists who use microscopes is just as important as the equipment itself.

“The University of Oslo began using electron microscopes early on. In the 1950s, the university was one of the first universities in the world to use TEM microscopes for brain research. We are probably the most frequent in the world. The electron microscopy laboratory cited. Neuroscience, “says the professor.

Over the past century, the Research Hub, also known as the Oslo School of Neuroscience, has nurtured celebrities such as polar explorers Fridtjof Nansen, senior Janyansen, Alfbrodal, Theodore Blackstud, and Per Andersen. The latter was the director of Nobel laureates Edwald and My Brit Moser.

You can see exactly where the cells are

A recent article by Nature Communications was initiated by researchers at the Karolinska Institute in Sweden. They wanted to test the hypothesis about the small cells that surround the smallest blood vessels in the brain. These cells play an important role in the formation of scars after a brain injury such as a stroke.

When these scars form, the brain has a hard time repairing damaged nerve fibers. A key factor in developing a drug to counter this is probably to prevent these cells from dividing and escaping through the walls of blood vessels. But Swedish scientists needed help with the very small details of their work.

“Mark” the cells with gold particles. The contribution to this article was to show the exact location of these cells belonging to a type of cell called pericytes. We are currently in the process of further characterizing these cells.These differences cell Pericytes that do not penetrate through the walls of blood vessels? Is it possible to distinguish only by appearance? “Amily Mogadam asks.

Christian Golitz, a scientist at the Karolinska Institute in Stockholm, would like to thank Scandinavia for their cooperation with Amily Mogadam.

“His laboratory has unique expertise that allowed us to characterize scar-forming pericytes at the hyperfine structure level. Professor Amry-Moghaddam, whose TEM microscope plays a central role. We have already started our next collaboration project with the group. We will expand our Nordic collaboration, “says Göritz.

The electron microscope is nearing the end of its life

Amiry-Moghaddam points out that microscopes contribute to hundreds of publications. But now they are between the ages of 13 and 19. As they grow older, teams must pay attention to how they use them.

“Previously, scientists were lining up and there was a waiting list, but now they can’t afford to be as open as they used to be. Those who use the equipment are familiar with how to use it. You need to be. You’re more likely to tolerate the error. With new equipment available, more people will be able to use it again, “he says.

The cost of each electron microscope is 6-7 million kronor, and there is little opportunity to apply for funding to replace such expensive equipment.

“At the same time, it is essential to maintain the expertise of our team, which is just as important as the equipment itself,” says the professor.

Artificial intelligence expands the usefulness of electron microscopy

For more information:
David O. Dias et al, Pericyte-derived fibrotic scars are conserved throughout a variety of central nervous system lesions. Nature Communications (2021). DOI: 10.1038 / s41467-021-25585-5

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University of Oslo

Quote: Https: // The smallest blood vessels in the brain obtained on January 14, 2022 (January 14, 2022) ) Examine the small cells surrounding

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