3D microscope quickly reveals the characteristics of living tissue

A team at Columbia University has created a 3D microscope that can be used to image living tissue. This technique helps clinicians identify tissue features without the need for histology or biopsy, and can be invaluable during surgery. In one application, the surgeon uses a scope to identify tumor boundaries during surgery without taking tissue samples for rapid histological analysis. This technology allows clinicians and researchers to use scopes to roam tissues and transform these data into 3D renderings of tissue structures. All do not damage the imaged structure.

There is a long tradition of removing tissues from the body to analyze them. Histological methods have evolved for a very long time and have been useful in modern medicine.But what if we could analyze a living tissue? thereDo you want to create a 3D model of the tissue structure that is almost real-time and even imaged? This approach is now enabled by new technologies that can blow many traditional histological practices out of the water.

“The method of processing biopsy samples has not changed for 100 years. It is cut out, fixed, embedded, sliced, dyed, placed on a glass slide and observed by a pathologist using a simple microscope. That’s why it can take several days to hear news about your diagnosis after a biopsy, “said Elizabeth Hillman, a researcher involved in the study.

MediSCAPE imaging of fresh human kidneys compared to gold standard periodated shiff (PAS) histology of the same tissue. In the center you will see a large area scanned using MediSCAPE. The panel shows certain characteristics of the kidney that represent normal or diseased. Excited autofluorescence at 488 nm is color coded on a yellow-blue scale.

Researchers said, “A miniaturized high-speed light sheet microscope. there “Volume histological imaging” allows you to obtain detailed histological images of living meat. Part of the success of this technique is the ability to quickly acquire images and the high sensitivity to detect small amounts of naturally occurring fluorescence in tissues. That is, no fluorescently labeled molecules or other stains are needed.

“One of the first tissues we examined was the kidneys of fresh mice. I was surprised to see a gorgeous structure similar to that obtained with standard histology,” he said. Another researcher, Kripa Patel, said. “Most importantly, we didn’t add dye to the mice. Everything we saw was natural fluorescence in the tissue, usually too weak to see. Microscopes are so efficient that they scan different areas of tissue as if they had a flashlight, imaging the entire 3D volume fast enough to move around in real time. I could see the weak signal of. “

Researchers hope that this technique will be especially useful for surgeons who want to examine tissue composition in real time. “Such technologies can provide real-time feedback on the type of tissue doctors are looking at without waiting for a long time,” says Hillman. “This immediate response allows us to make informed decisions about the best way to remove a tumor and make sure it is not left behind.”

See 3D rendering from the microscope below.

Above image: MediSCAPE image of a living human tongue. Subject slowly licked the MediSCAPE imaging head while acquiring high-speed 3D images. Then the volume was stitched to form a long 3D strip underneath. Contrast shows red and green autofluorescence with excitation at 488 nm. The inset on the left shows the MediSCAPE imaging geometry, which uses a diagonal light sheet to illuminate the sample and scan left and right to quickly capture a 3D image. The inset shows the concept of a handheld MediSCAPE system for use in surgery.

Study at Nature Biomedical Engineering: High-speed light sheet microscope for acquiring volumetric histological images of living tissue on the spot

Via: Columbia University 3D microscope quickly reveals the characteristics of living tissue

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